--------------------------------------------------------------------------------
|             Helix Systems -- Center for Information Technology               |
|                            http://helix.nih.gov                              |
|   National Institutes of Health, Department of Health and Human Services     |
|                                                                              |
|             DNAWorks Web Site: http://helixweb.nih.gov/dnaworks              |
--------------------------------------------------------------------------------
|                                                                              |
|              Send all correspondence to webtools@helix.nih.gov               |
--------------------------------------------------------------------------------

 Job started on 09/03/2013 at 14:40:38

 Job name: NW9022013TBP2B                                                       

 Output will be sent to nicole_welch23@yahoo.com      
 
      SEQUENCE  1: PROTEIN LENGTH =  431                               
 ----------------------------------------------------------------
   1 MSSGASHHELTRGRDGLKEREYVWKKSHADESPGSNPQILPTLVLPHHLVFDNDGAPLAD
  61 NIKVHFGRGWRLHVEDALNIVHRCALIMKEEPNVVRLKGSAVVCGDLHGQFHDLLTLLEV
 121 NGHPSVQQYVFLGDYVDRGDFSAEIVLLCMSFKLLYPRSFILLRGNHESRQLTSCFNFKQ
 181 EIESKYSSMVYEEIMAAFDCFPLSCVVNDRFFCVHGGLSPLLTYLGEIDTVNRFRETPST
 241 GPMCDLLWSDPMFGDDTDCATPSEELFVFNTKRGCSYNYSYEAVCRFLEANNLCTVIRGH
 301 ETQPGGYKLYRHTPKGVPAVVCVFSASNYCGTYGNMAAVVAIDGDVMNIRQYMATSHDSC
 361 TPNHFNAISRMQPLAIHEAVEKWCVASHGGSSGDAKAEKVEVEKNLSEDDSSVVLREKLG
 421 DMICAMHHIVT
 ----------------------------------------------------------------
 
             CODON FREQUENCY TABLE: E. coli Class II              
 ----------------------------------------------------------------
 
      TTT F 0.291   TCT S 0.324   TAT Y 0.352   TGT C 0.389
      TTC F 0.709   TCC S 0.266   TAC Y 0.648   TGC C 0.612
      TTA L 0.034   TCA S 0.048   TAA X 0.630   TGA X 0.352
      TTG L 0.055   TCG S 0.074   TAG X 0.076   TGG W 1.000
 
      CTT L 0.056   CCT P 0.112   CAT H 0.298   CGT R 0.643
      CTC L 0.080   CCC P 0.016   CAC H 0.702   CGC R 0.330
      CTA L 0.008   CCA P 0.153   CAA Q 0.187   CGA R 0.011
      CTG L 0.767   CCG P 0.719   CAG Q 0.814   CGG R 0.008
 
      ATT I 0.335   ACT T 0.291   AAT N 0.173   AGT S 0.045
      ATC I 0.659   ACC T 0.536   AAC N 0.828   AGC S 0.243
      ATA I 0.006   ACA T 0.047   AAA K 0.786   AGA R 0.006
      ATG M 1.000   ACG T 0.127   AAG K 0.215   AGG R 0.003
 
      GTT V 0.398   GCT A 0.275   GAT D 0.461   GGT G 0.508
      GTC V 0.135   GCC A 0.161   GAC D 0.540   GGC G 0.428
      GTA V 0.200   GCA A 0.240   GAA E 0.754   GGA G 0.020
      GTG V 0.268   GCG A 0.323   GAG E 0.247   GGG G 0.044
 
 
             ACTIVE CODONS FOR SEQUENCE GENERATION 
 
  Residue   Codons   Active Codons     Min. Freq.    Max. Freq.
 ----------------------------------------------------------------
   A  Ala      4           4             0.161         0.323
   C  Cys      2           2             0.389         0.612
   D  Asp      2           2             0.461         0.540
 
   E  Glu      2           2             0.247         0.754
   F  Phe      2           2             0.291         0.709
   G  Gly      4           2             0.428         0.508
 
   H  His      2           2             0.298         0.702
   I  Ile      3           2             0.335         0.659
   K  Lys      2           2             0.215         0.786
 
   L  Leu      6           2             0.080         0.767
   M  Met      1           1             1.000         1.000
   N  Asn      2           2             0.173         0.828
 
   P  Pro      4           3             0.112         0.719
   Q  Gln      2           2             0.187         0.814
   R  Arg      6           2             0.330         0.643
 
   S  Ser      6           3             0.243         0.324
   T  Thr      4           3             0.127         0.536
   V  Val      4           4             0.135         0.398
 
   W  Trp      1           1             1.000         1.000
   X  End      3           2             0.352         0.630
   Y  Tyr      2           2             0.352         0.648
 
 
                SEQUENCE PATTERNS TO BE SCREENED
 ----------------------------------------------------------------
 ----------------------------------------------------------------
 
 ----------------------------------------------------------------
                    PARAMETERS FOR TRIAL 1                             
 ----------------------------------------------------------------
               Total Size Of Gene ......... 1293 nt                       
               Protein Residues ........... 431                           
               Mutatable Residues ......... 414                           
               Fixed Nucleotides .......... 51 nt                         
               Degenerate Nucleotides ..... 0 nt                          
               Oligo Size ................. 60 nt                         
               Annealing Temp ............. 62 +/- 1*C                    
               Oligo Concentration ........ 1.00E-7 M                     
               Sodium Concentration ....... 5.00E-2 M                     
               Mg2+ Concentration ......... 2.00E-3 M                     
               Codon Frequency Threshold .. 10%                           
               Repeat Threshold ........... 8 nt                          
               Mispriming Threshold ....... 8/18 (6 exact) nt             
 
--------------------------------------------------------------------------------
 
 The DNA sequence #   1 is:
 ----------------------------------------------------------------
   1 ATGTCTTCTGGCGCGTCCCATCATGAGCTGACCCGTGGTCGTGATGGTCTCAAAGAACGT
  61 GAATACGTTTGGAAAAAATCTCACGCGGACGAGTCCCCGGGTTCTAACCCGCAAATCCTG
 121 CCGACCCTGGTTCTGCCGCACCACCTCGTGTTCGACAACGACGGTGCGCCACTGGCGGAC
 181 AACATCAAAGTTCACTTCGGTCGCGGTTGGCGCCTCCACGTTGAGGATGCGCTGAATATC
 241 GTTCACCGTTGCGCGCTGATCATGAAGGAGGAACCGAATGTTGTACGCCTGAAGGGCTCT
 301 GCAGTAGTCTGCGGTGATCTCCACGGCCAGTTCCACGACCTCCTGACCCTGCTGGAAGTT
 361 AATGGTCACCCTAGCGTTCAACAGTACGTTTTCCTGGGTGACTACGTTGACCGTGGTGAC
 421 TTCTCTGCTGAAATCGTTCTGCTGTGCATGTCTTTCAAACTGCTGTACCCGCGCTCTTTC
 481 ATCCTGCTGCGTGGTAACCACGAGTCTCGCCAGCTGACGTCTTGCTTCAACTTCAAACAG
 541 GAAATCGAATCTAAATACTCTTCTATGGTTTACGAAGAAATCATGGCGGCGTTCGACTGC
 601 TTCCCGCTGTCTTGCGTTGTTAACGACCGTTTCTTTTGTGTTCATGGTGGTCTCTCTCCG
 661 CTGCTGACCTACCTCGGTGAGATCGATACTGTTAACCGTTTTCGTGAAACCCCGTCTACT
 721 GGTCCGATGTGTGACCTGCTCTGGTCTGATCCAATGTTCGGTGATGACACCGACTGTGCG
 781 ACGCCGAGCGAAGAACTGTTCGTTTTTAACACTAAACGTGGTTGCTCTTACAACTACAGC
 841 TACGAAGCCGTTTGCCGTTTCCTCGAAGCGAACAATCTGTGCACGGTTATTCGTGGTCAT
 901 GAGACCCAGCCGGGTGGTTATAAACTGTACCGCCATACCCCAAAGGGTGTTCCGGCGGTT
 961 GTATGTGTTTTTAGCGCGAGCAATTATTGCGGTACCTACGGTAACATGGCCGCAGTTGTA
1021 GCGATCGATGGTGACGTTATGAACATCCGTCAGTACATGGCGACCTCTCACGACTCTTGC
1081 ACCCCTAACCACTTCAATGCGATCTCTCGTATGCAGCCTCTGGCGATCCATGAGGCGGTA
1141 GAAAAGTGGTGTGTTGCGTCTCACGGTGGTTCTTCCGGTGACGCGAAAGCGGAAAAAGTT
1201 GAGGTTGAAAAAAACCTGTCTGAAGATGACAGCTCTGTTGTTCTGCGTGAAAAACTGGGC
1261 GATATGATCTGCGCAATGCACCACATTGTTACC
 ----------------------------------------------------------------
 
 The oligonucleotide assembly is:
 ----------------------------------------------------------------
     1       10        20        30        40        50        60
     |        |         |         |         |         |         |
 
     1 --->                                                      
   1 ATGTCTTCTGGCGCGTCCCATCATGAGCTGACCCGTGGTCGTGAT               
                                 ACTGGGCACCAGCACTACCAGAGTTTCTTGCA
                                                                 
      M  S  S  G  A  S  H  H  E  L  T  R  G  R  D  G  L  K  E  R 
 
     |        |         |         |         |         |         |
 
            3 --->                                               
  61        tttggaaaaaatctcacgcggacgagtccccgggttctaacccgcaaatcctg
     CTTATGCAAACCTTTTTTAGAGTGCGCC                      cgtttaggac
                          <---  2                                
      E  Y  V  W  K  K  S  H  A  D  E  S  P  G  S  N  P  Q  I  L 
 
     |        |         |         |         |         |         |
 
                                        5 --->                   
 121 ccgaccc                            CAACGACGGTGCGCCACTGGCGGAC
     ggctgggaccaagacggcgtggtggagcacaagctgttgctgccacgcgg          
                                                <---  4          
      P  T  L  V  L  P  H  H  L  V  F  D  N  D  G  A  P  L  A  D 
 
     |        |         |         |         |         |         |
 
                                                                 
 181 AACATCAAAGTTCACTTCGGTCGCGGTTGGCGCCT                         
                          GCGCCAACCGCGGAGGTGCAACTCCTACGCGACTTATAG
                                                                 
      N  I  K  V  H  F  G  R  G  W  R  L  H  V  E  D  A  L  N  I 
 
     |        |         |         |         |         |         |
 
          7 --->                                                 
 241      ccgttgcgcgctgatcatgaaggaggaaccgaatgttgtacgcctgaagggctct
     CAAGTGGCAACGCGCGACTAG                          ggacttcccgaga
                   <---  6                                       
      V  H  R  C  A  L  I  M  K  E  E  P  N  V  V  R  L  K  G  S 
 
     |        |         |         |         |         |         |
 
                                 9 --->                          
 301 gcagt                       AGTTCCACGACCTCCTGACCCTGCTGGAAGTT
     cgtcatcagacgccactagaggtgccggtcaaggtgctggaggactg             
                                             <---  8             
      A  V  V  C  G  D  L  H  G  Q  F  H  D  L  L  T  L  L  E  V 
 
     |        |         |         |         |         |         |
 
                                                       11 --->   
 361 AATGGTCACCCTAGCGTTCAACAGTACG                      ccgtggtgac
             GGGATCGCAAGTTGTCATGCAAAAGGACCCACTGATGCAACTGGCACCACTG
                                                                 
      N  G  H  P  S  V  Q  Q  Y  V  F  L  G  D  Y  V  D  R  G  D 
 
     |        |         |         |         |         |         |
 
                                                                 
 421 ttctctgctgaaatcgttctgctgtgcatgtctttcaaactgctgtaccc          
     AAGAGACG                     cagaaagtttgacgacatgggcgcgagaaag
      <--- 10                                                    
      F  S  A  E  I  V  L  L  C  M  S  F  K  L  L  Y  P  R  S  F 
 
     |        |         |         |         |         |         |
 
               13 --->                                           
 481           GTGGTAACCACGAGTCTCGCCAGCTGACGTCTTGCTTCAACTTCAAACAG
     taggacgacgcaccattggtgctcagagc                 AGTTGAAGTTTGTC
                           <--- 12                               
      I  L  L  R  G  N  H  E  S  R  Q  L  T  S  C  F  N  F  K  Q 
 
     |        |         |         |         |         |         |
 
                          15 --->                                
 541 GAAATCGAAT           tctatggtttacgaagaaatcatggcggcgttcgactgc
     CTTTAGCTTAGATTTATGAGAAGATACCAAATGCTTCTTTAGTACC              
                                            <--- 14              
      E  I  E  S  K  Y  S  S  M  V  Y  E  E  I  M  A  A  F  D  C 
 
     |        |         |         |         |         |         |
 
                                                  17 --->        
 601 ttcccgctgtcttgcgttgtt                        GGTGGTCTCTCTCCG
        ggcgacagaacgcaacaattgctggcaaagaaaacacaagtaccaccagagagaggc
                                                             <---
      F  P  L  S  C  V  V  N  D  R  F  F  C  V  H  G  G  L  S  P 
 
     |        |         |         |         |         |         |
 
                                                                 
 661 CTGCTGACCTACCTCGGTGAGATCGATACTGTTAACCGTTTTCGT               
     gac                    GCTATGACAATTGGCAAAAGCACTTTGGGGCAGATGA
      16                                                         
      L  L  T  Y  L  G  E  I  D  T  V  N  R  F  R  E  T  P  S  T 
 
     |        |         |         |         |         |         |
 
         19 --->                                                 
 721     cgatgtgtgacctgctctggtctgatccaatgttcggtgatgacaccgactgtgcg
     CCAGGCTACACACTGGACGAGAC                         tggctgacacgc
                     <--- 18                                     
      G  P  M  C  D  L  L  W  S  D  P  M  F  G  D  D  T  D  C  A 
 
     |        |         |         |         |         |         |
 
                                21 --->                          
 781 acgc                       AACACTAAACGTGGTTGCTCTTACAACTACAGC
     tgcggctcgcttcttgacaagcaaaaattgtgatttgcaccaacgaga            
                                              <--- 20            
      T  P  S  E  E  L  F  V  F  N  T  K  R  G  C  S  Y  N  Y  S 
 
     |        |         |         |         |         |         |
 
                                                      23 --->    
 841 TACGAAGCCGTTTGCCGTTTCCTCGAA                      ttcgtggtcat
             GCAAACGGCAAAGGAGCTTCGCTTGTTAGACACGTGCCAATAAGCACCAGTA
                                                                 
      Y  E  A  V  C  R  F  L  E  A  N  N  L  C  T  V  I  R  G  H 
 
     |        |         |         |         |         |         |
 
                                                                 
 901 gagacccagccgggtggttataaactgtaccgccataccccaaagggtg           
     CTCTGGGT                       cggtatggggtttcccacaaggccgccaa
      <--- 22                                                    
      E  T  Q  P  G  G  Y  K  L  Y  R  H  T  P  K  G  V  P  A  V 
 
     |        |         |         |         |         |         |
 
                  25 --->                                        
 961              GCGCGAGCAATTATTGCGGTACCTACGGTAACATGGCCGCAGTTGTA
     catacacaaaaatcgcgctcgttaataacgc                      TCAACAT
                             <--- 24                             
      V  C  V  F  S  A  S  N  Y  C  G  T  Y  G  N  M  A  A  V  V 
 
     |        |         |         |         |         |         |
 
                                        27 --->                  
1021 GCGATCGATGGTG                      catggcgacctctcacgactcttgc
     CGCTAGCTACCACTGCAATACTTGTAGGCAGTCATGTACCGCTGGAGAGTGCT       
                                                   <--- 26       
      A  I  D  G  D  V  M  N  I  R  Q  Y  M  A  T  S  H  D  S  C 
 
     |        |         |         |         |         |         |
 
                                                           29 ---
1081 acccctaaccacttcaatgcgatctctcgtatgca                   GCGGTA
                   gttacgctagagagcatacgtcggagaccgctaggtactccgccat
                                                                 
      T  P  N  H  F  N  A  I  S  R  M  Q  P  L  A  I  H  E  A  V 
 
     |        |         |         |         |         |         |
 
     >                                                           
1141 GAAAAGTGGTGTGTTGCGTCTCACGGTGGTTCTTCCGGTGACGCGAAAGCGGAA      
     cttttcaccacaca                       CACTGCGCTTTCGCCTTTTTCAA
            <--- 28                                              
      E  K  W  C  V  A  S  H  G  G  S  S  G  D  A  K  A  E  K  V 
 
     |        |         |         |         |         |         |
 
                     31 --->                                     
1201                 tgtctgaagatgacagctctgttgttctgcgtgaaaaactgggc
     CTCCAACTTTTTTTGGACAGACTTCTACTGTCGAGAC                    ccg
                                   <--- 30                       
      E  V  E  K  N  L  S  E  D  D  S  S  V  V  L  R  E  K  L  G 
 
     |        |         |         |         |         |         |
 
                                      
1261 gatatgatctgcgcaa                 
     ctatactagacgcgttacgtggtgtaacaatgg
                               <--- 32
      D  M  I  C  A  M  H  H  I  V  T 
 ----------------------------------------------------------------
 
 The total codon usage score ...........     0.000
 The total length score ................     0.000
 The total melting temperature score ...     0.000
 The total repeat score ................     0.000
 The total pattern score ...............     0.000
 The total mispriming score ............     0.000
 The total AT content score ............     0.000
 The total GC content score ............     0.000
 The total fixed gap score .............      N/A
                The OVERALL score ......     0.000
 
 
                  DETAILED CODON FREQUENCY REPORT 
  [ Codon, AA, Frequency, # of times used in coding sequence ] 
 ----------------------------------------------------------------
 
 TTT F 0.29   4  TCT S 0.32  25  TAT Y 0.35   2  TGT C 0.39   5
 TTC F 0.71  16  TCC S 0.27   3  TAC Y 0.65  13  TGC C 0.61  12
 TTA L 0.03   0  TCA S 0.05   0  TAA X 0.63   0  TGA X 0.35   0
 TTG L 0.06   0  TCG S 0.07   0  TAG X 0.08   0  TGG W 1.00   4
 
 CTT L 0.06   0  CCT P 0.11   3  CAT H 0.30   6  CGT R 0.64  15
 CTC L 0.08   9  CCC P 0.02   0  CAC H 0.70  15  CGC R 0.33   6
 CTA L 0.01   0  CCA P 0.15   3  CAA Q 0.19   2  CGA R 0.01   0
 CTG L 0.77  30  CCG P 0.72  13  CAG Q 0.81   7  CGG R 0.01   0
 
 ATT I 0.34   2  ACT T 0.29   3  AAT N 0.17   6  AGT S 0.05   0
 ATC I 0.66  14  ACC T 0.54  12  AAC N 0.83  14  AGC S 0.24   6
 ATA I 0.01   0  ACA T 0.05   0  AAA K 0.79  13  AGA R 0.01   0
 ATG M 1.00  13  ACG T 0.13   3  AAG K 0.22   4  AGG R 0.00   0
 
 GTT V 0.40  30  GCT A 0.28   1  GAT D 0.46   9  GGT G 0.51  27
 GTC V 0.14   1  GCC A 0.16   2  GAC D 0.54  17  GGC G 0.43   4
 GTA V 0.20   5  GCA A 0.24   3  GAA E 0.75  19  GGA G 0.02   0
 GTG V 0.27   1  GCG A 0.32  20  GAG E 0.25   9  GGG G 0.04   0
 
             
              Frequency Range   Number of Codons
             -------------------------------------
                0% -  4%                  0
                5% -  9%                  9
               10% - 14%                  7
               15% - 19%                 13
               20% - 24%                 27
               25% - 29%                 18
               30% - 34%                 53
               35% - 39%                 37
               40% - 44%                  4
               45% - 49%                  9
                  >= 50%                254
             -------------------------------------
              Total Codons Used =   431
 
                  Tm Range    # of Overlaps 
             -------------------------------------
                   <60            0
                    60            0
                    61           11
                    62           20
                    63            0
                    64            0
                    65            0
                    66            0
                    67            0
                  >=68            0
             -------------------------------------
              Tm Range =  1.9
 
              Ovrlap Len Range  # of Oligos
             -------------------------------------
                   <15            1
                    15            1
                    16            2
                    17            3
                    18            7
                    19            6
                    20            3
                    21            5
                    22            1
                    23            0
                    24            1
                  >=25            1
             -------------------------------------
              Lowest Overlap = 14
 
              Length Range   # of Oligos
             -------------------------------------
                   < 50            2
                   50- 51           0
                   52- 53           0
                   54- 55           0
                   56- 57           0
                   58- 59           0
                   60- 61          30
                   62- 63           0
                   64- 65           0
                   66- 67           0
                   68- 69           0
                   >= 70           0
             -------------------------------------
              Longest =  60
 
 
          Sequence Patterns Screened (As Supplied By User)
 ----------------------------------------------------------------
                       None found
 ----------------------------------------------------------------
 
        32 oligonucleotides need to be synthesized
 ----------------------------------------------------------------
  1 ATGTCTTCTGGCGCGTCCCATCATGAGCTGACCCGTGGTCGTGAT  45
  2 CCGCGTGAGATTTTTTCCAAACGTATTCACGTTCTTTGAGACCATCACGACCACGGGTCA  60
  3 TTTGGAAAAAATCTCACGCGGACGAGTCCCCGGGTTCTAACCCGCAAATCCTGCCGACCC  60
  4 GGCGCACCGTCGTTGTCGAACACGAGGTGGTGCGGCAGAACCAGGGTCGGCAGGATTTGC  60
  5 CAACGACGGTGCGCCACTGGCGGACAACATCAAAGTTCACTTCGGTCGCGGTTGGCGCCT  60
  6 GATCAGCGCGCAACGGTGAACGATATTCAGCGCATCCTCAACGTGGAGGCGCCAACCGCG  60
  7 CCGTTGCGCGCTGATCATGAAGGAGGAACCGAATGTTGTACGCCTGAAGGGCTCTGCAGT  60
  8 GTCAGGAGGTCGTGGAACTGGCCGTGGAGATCACCGCAGACTACTGCAGAGCCCTTCAGG  60
  9 AGTTCCACGACCTCCTGACCCTGCTGGAAGTTAATGGTCACCCTAGCGTTCAACAGTACG  60
 10 GCAGAGAAGTCACCACGGTCAACGTAGTCACCCAGGAAAACGTACTGTTGAACGCTAGGG  60
 11 CCGTGGTGACTTCTCTGCTGAAATCGTTCTGCTGTGCATGTCTTTCAAACTGCTGTACCC  60
 12 CGAGACTCGTGGTTACCACGCAGCAGGATGAAAGAGCGCGGGTACAGCAGTTTGAAAGAC  60
 13 GTGGTAACCACGAGTCTCGCCAGCTGACGTCTTGCTTCAACTTCAAACAGGAAATCGAAT  60
 14 CCATGATTTCTTCGTAAACCATAGAAGAGTATTTAGATTCGATTTCCTGTTTGAAGTTGA  60
 15 TCTATGGTTTACGAAGAAATCATGGCGGCGTTCGACTGCTTCCCGCTGTCTTGCGTTGTT  60
 16 CAGCGGAGAGAGACCACCATGAACACAAAAGAAACGGTCGTTAACAACGCAAGACAGCGG  60
 17 GGTGGTCTCTCTCCGCTGCTGACCTACCTCGGTGAGATCGATACTGTTAACCGTTTTCGT  60
 18 CAGAGCAGGTCACACATCGGACCAGTAGACGGGGTTTCACGAAAACGGTTAACAGTATCG  60
 19 CGATGTGTGACCTGCTCTGGTCTGATCCAATGTTCGGTGATGACACCGACTGTGCGACGC  60
 20 AGAGCAACCACGTTTAGTGTTAAAAACGAACAGTTCTTCGCTCGGCGTCGCACAGTCGGT  60
 21 AACACTAAACGTGGTTGCTCTTACAACTACAGCTACGAAGCCGTTTGCCGTTTCCTCGAA  60
 22 TGGGTCTCATGACCACGAATAACCGTGCACAGATTGTTCGCTTCGAGGAAACGGCAAACG  60
 23 TTCGTGGTCATGAGACCCAGCCGGGTGGTTATAAACTGTACCGCCATACCCCAAAGGGTG  60
 24 CGCAATAATTGCTCGCGCTAAAAACACATACAACCGCCGGAACACCCTTTGGGGTATGGC  60
 25 GCGCGAGCAATTATTGCGGTACCTACGGTAACATGGCCGCAGTTGTAGCGATCGATGGTG  60
 26 TCGTGAGAGGTCGCCATGTACTGACGGATGTTCATAACGTCACCATCGATCGCTACAACT  60
 27 CATGGCGACCTCTCACGACTCTTGCACCCCTAACCACTTCAATGCGATCTCTCGTATGCA  60
 28 ACACACCACTTTTCTACCGCCTCATGGATCGCCAGAGGCTGCATACGAGAGATCGCATTG  60
 29 GCGGTAGAAAAGTGGTGTGTTGCGTCTCACGGTGGTTCTTCCGGTGACGCGAAAGCGGAA  60
 30 CAGAGCTGTCATCTTCAGACAGGTTTTTTTCAACCTCAACTTTTTCCGCTTTCGCGTCAC  60
 31 TGTCTGAAGATGACAGCTCTGTTGTTCTGCGTGAAAAACTGGGCGATATGATCTGCGCAA  60
 32 GGTAACAATGTGGTGCATTGCGCAGATCATATCGCC  36
 
                          FINAL SUMMARY FOR   1 SOLUTION   
--------------------------------------------------------------------------------
  #    Tm   Len  |    Score   TmRange  Short    Long   #Olig  #Repeat #Misprime
 
  1    62    60  |    0.000       1.9      14      60      32       0       0
 
--------------------------------------------------------------------------------
|             Helix Systems -- Center for Information Technology               |
|                            http://helix.nih.gov                              |
|   National Institutes of Health, Department of Health and Human Services     |
|                                                                              |
|             DNAWorks Web Site: http://helixweb.nih.gov/dnaworks              |
--------------------------------------------------------------------------------

